abhd17a

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

The expression of ABHD17a resulted in a larger and more consistent reduction in membrane expression of the STREX–CRD (stress-regulated exon) fusion protein compared with ABHD17c

ABHD17a (α/β-hydrolase domain-containing protein 17a) deacylates the stress-regulated exon domain of large conductance voltage- and calcium-activated potassium (BK) channels inhibiting channel activity independently of effects on channel surface expression. 

It suggests that overexpression of the catalytically null ABHD17a has distinct effects on the trafficking pathways controlling ZERO and STREX variant surface expression.

We can use a membrane potential assay in HEK293 cells in which heterologously expressed BK channels are stimulated upon calcium entry mediated through the calcium ionophore ionomycin.

It ubiquitous expression in spleen and bone marrow.

Constructing a palmitoylation-deficient mutant [ABHD17B-5CS (C10,11,14,15,18S)] and a myristoylated ABHD17B mutant (N-myr-ABHD17B), in which an N-terminal palmitoylation motif was replaced with a myristoylation motif of Src; Both mutants lost the depalmitoylating activity toward PSD-95. These results raise the possibility that the enzymatic activity of ABHD17A depends on its own palmitoylation.

Negative regulation of leukocyte mediated cytotoxicity; Negative regulation of cell killing.

CSNK1G2, MIB2, and TMEM259.

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit.