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MYLK

Official Full Name

myosin light chain kinase
Overview

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3 region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
Synonyms

MYLK; myosin light chain kinase; myosin, light polypeptide kinase; myosin light chain kinase, smooth muscle; MLCK; MLCK1; MYLK1; smMLCK; smooth muscle myosin light chain kinase; telokin; kinase-related protein; KRP; AAT7; MLCK108; MLCK210; MSTP083; FLJ122;

Type

Recombinant Proteins
Cell & Tissue Lysates

Species

Chicken
Human
Mouse

Source

E.coli
HEK293
HEK293T
In Vitro Cell Free System
Insect Cell
Mammalian Cell
Sf9 Insect Cell
Wheat Germ

Tag

GST
His
His|GST
His|T7
SUMO
Myc
DDK
MYC
N/A
N

Species	Cat.#	Product name	Source (Host)	Tag	Protein Length	Price
Human	MYLK-1509H	Recombinant Human MYLK protein, His & GST-tagged	E.coli	His/GST	Phe1661~Asp1877 (Accession # Q15746)	Inquiry
Human	MYLK-696H	Recombinant Human Myosin Light Chain Kinase, GST-tagged	Insect Cell	GST		Inquiry
Human	MYLK-27838TH	Recombinant Human MYLK		N/A		Inquiry
Human	MYLK-355H	Recombinant Human Myosin Light Chain Kinase, GST-tagged, Active	Sf9 Insect Cell	GST		Inquiry
Human	MYLK-4017HCL	Recombinant Human MYLK 293 Cell Lysate	HEK293	N/A		Inquiry
Human	MYLK-4858H	Recombinant Human MYLK Protein, Myc/DDK-tagged, C13 and N15-labeled	HEK293T	Myc/DDK		Inquiry
Human	MYLK-5820H	Recombinant Human MYLK Protein, GST-tagged	Wheat Germ	GST		Inquiry
Human	MYLK-5954H	Recombinant Human MYLK protein, His-tagged	E.coli	His	1464-1719aa	Inquiry
Human	MYLK-6768HF	Recombinant Full Length Human MYLK Protein, GST-tagged	In Vitro Cell Free System	GST	992 amino acids	Inquiry
Human	MYLK-4651H	Recombinant Human MYLK Protein (Thr1748-Glu1914), N-His tagged	E.coli	N-His	Thr1748-Glu1914	Inquiry
Mouse	Mylk-1510M	Recombinant Mouse Mylk protein, His & T7-tagged	E.coli	His/T7	Met1684~Asp1884 (Accession # Q6PDN3)	Inquiry
Mouse	Mylk-335M	Recombinant Mouse Mylk Protein, MYC/DDK-tagged	HEK293T	MYC/DDK		Inquiry
Chicken	MYLK-7009C	Recombinant Chicken MYLK	Mammalian Cell	His		Inquiry
Chicken	Mylk-3878C	Recombinant Chicken Mylk protein, His-SUMO & Myc-tagged	E.coli	His-SUMO & Myc	1435-1722aa	Inquiry

Involved Pathway
Protein Function
Interacting Protein

MYLK involved in several pathways and played different roles in them. We selected most pathways MYLK participated on our site, such as Calcium signaling pathway, cGMP-PKG signaling pathway, Vascular smooth muscle contraction, which may be useful for your reference. Also, other proteins which involved in the same pathway with MYLK were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name	Pathway Related Protein
Calcium signaling pathway	DRD7;CALML5;ATP2B1;EDNRA;CALM1B;P2RX8;PLCD3;ADRB3B;EDNRAA
cGMP-PKG signaling pathway	IRS4;PRKG1;ATP1A2;PRKG2;IRS2;CALML5;SLC25A6;MYLK;BDKRB2
Vascular smooth muscle contraction	ADORA2AA;ADCY1B;MYL12.1;MYLK4;PPP1R14AA;CALM1;KCNMA1A;MYLK;PRKG1A
Focal adhesion	LAMA2;PIK3R3B;KDR;PPP1CA;COL6A5;KDRL;RHOAB;PAK6B;ITGA6
Platelet activation	GUCY1A3;COL24A1;FCGR2A;MYLK4;MYL12A;ROCK2;PIK3R1;GNAS;PRKACB
Regulation of actin cytoskeleton	INSRR;ITGA4;FGF11A;FGF20B;ITGA6;MSN;FGF7;RAF1;RRAS2
Oxytocin signaling pathway	CAMK2B;PPP3R2;GNAI2;PLCB1;CAMK2G;CAMK2D;CACNB1;PTGS2;PIK3R3
Gastric acid secretion	CAMK2D;SLC9A4;KCNJ1;ITPR3;ATP1A4;Car2;MYLK4;PLCB3;ACTB

MYLK has several biochemical functions, for example, ATP binding, actin binding, calmodulin binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MYLK itself. We selected most functions MYLK had, and list some proteins which have the same functions with MYLK. You can find most of the proteins on our site.

Function	Related Protein
ATP binding	NLRP5;TRPM6;MYHZ2;ATAD5;STK32C;ITPKB;ULK1A;NIM1K;MYO19
actin binding	PXK;LRRC10;EPB49;XIRP1;CAPGA;PARVA;MYHC4;WIPF1A;PFN3
calmodulin binding	SNTA1;CAMK1;SNTB1;NOS3;MYO1G;ATPIF1;SCN5A;MYO5A;CNN3
metal ion binding	CYP4A14;ZNF41;HIC2;IKZF1;ENPP4;SOBPB;ZNF75A;AGMAT;CCC2
myosin light chain kinase activity	MYLK3;MYLK2;MYLK
protein binding	TRAM2;E4F1;NEIL2;AKAP8L;DYNC1I2;DOCK2;MYD88;CCNDBP1;CENPL

MYLK has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MYLK here. Most of them are supplied by our site. Hope this information will be useful for your research of MYLK.

GOPC; MYL12A; NCK1; PIK3R1; GRB2; CTTN; HSPB1; q7ard3_yerpe; q7cje7_yerpe; ahpC; exuR; tbpA1; q8d0g3_yerpe; npm_alk