| Description : |
INK3, also known as SAPK 1b (Stress-Activated Protein Kinase 1b), is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
3.224mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, Frozen solution. |
| Bio-activity : |
As provided, this lot demonstrated <3.5% of maximum activity. Activated by phosphorylation with MKK4, active and MKK7β1, active. |
| Molecular Mass : |
53 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMGSPG ISGVDVSSVA KHYNMSKSKV DNQFYSVEVG 61 DSTFTVLKRY QNLKPIGSGA QGIVCAAYDA VLDRNVAIKK LSRPFQNQTH AKRAYRELVL 121 MKCVNHKNII SLLNVFTPQK TLEEFQDVYL VMELMDANLC QVIQMELDHE RMSYLLYQML 181 CGIKHLHSAG IIHRDLKPSN IVVKSDCTLK ILDFGLARTA GTSFMMTPYV VTRYYRAPEV 241 ILGMGYKENV DIWSVGCIMG EMVRHKILFP GRDYIDQWNK VIEQLGTPCP EFMKKLQPTV 301 RNYVENRPKY AGLTFPKLFP DSLFPADSEH NKLKASQARD LLSKMLVIDP AKRISVDDAL 361 QHPYINVWYD PAEVEAPPPQ IYDKQLDERE HTIEEWKELI YKEVMNSEEK TKNGVVKGQP 421 SPSGAAVNSS ESLPPSSSVN DISSMSTDQT LASDTDSSLE ASAGPLGCCR |
| Purity : |
Purity 97.2% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at - 70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
