Unactive Recombinant Full Length Human MAPK11 Protein, N-GST-tagged

• Cat.No.: MAPK11-122HFL
• Product Overview: N-terminal GST-tagged, recombinant, full-length, human p38 beta 2/SAPK2b, unactive, expressed in E.coli cells. Purified using glutathione-agarose.

Specification

• Description: MAPK11 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK11 functions are mostly redundant with those of MAPK14. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 2.74mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Liquid at -20 centigrade.
• Bio-activity: Asnprovided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with DDMKK6.
• Molecular Mass: 71 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM SGPRAGFYRQ 241 ELNKTVWEVP QRLQGLRPVG SGAYGSVCSA YDARLRQKVA VKKLSRPFQS LIHARRTYRE 301 LRLLKHLKHE NVIGLLDVFT PATSIEDFSE VYLVTTLMGA DLNNIVKCQA LSDEHVQFLV 361 YQLLRGLKYI HSAGIIHRDL KPSNVAVNED CELRILDFGL ARQADEEMTG YVATRWYRAP 421 EIMLNWMHYN QTVDIWSVGC IMAELLQGKA LFPGSDYIDQ LKRIMEVVGT PSPEVLAKIS 481 SEHARTYIQS LPPMPQKDLS SIFRGANPLA IDLLGRMLVL DSDQRVSAAE ALAHAYFSQY 541 HDPEDEPEAE PYDESVEAKE RTLEEWKELT YQEVLSFKPP EPPKPPGSLE IEQ
• Purity: Purity 97% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAPK11 mitogen-activated protein kinase 11 [ Homo sapiens (human) ]
• Official Symbol: MAPK11
• Synonyms: MAPK11; mitogen-activated protein kinase 11; P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
• Gene ID: 5600
• mRNA Refseq: NM_002751.7
• Protein Refseq: NP_002742.3
• MIM: 602898
• UniProt ID: Q15759