| Description : |
p38α/SAPK2a Protein is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP Kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
Full Length |
| Form : |
1.934mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Liquid at -20 centigrade. |
| Bio-activity : |
As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with DDMKK6. |
| Molecular Mass : |
67.7 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMSQE RPTFYRQELN 241 KTIWEVPERY QNLSPVGSGA YGSVCAAFDT KTGLRVAVKK LSRPFQSIIH AKRTYRELRL 301 LKHMKHENVI GLLDVFTPAR SLEEFNDVYL VTHLMGADLN NIVKCQKLTD DHVQFLIYQI 361 LRGLKYIHSA DIIHRDLKPS NLAVNEDCEL KILDFGLARH TDDEMTGYVA TRWYRAPEIM 421 LNWMHYNQTV DIWSVGCIMA ELLTGRTLFP GTDHIDQLKL ILRLVGTPGA ELLKKISSES 481 ARNYIQSLTQ MPKMNFANVF IGANPLAVDL LEKMLVLDSD KRITAAQALA HAYFAQYHDP 541 DDEPVADPYD QSFESRDLLI DEWKSLTYDE VISFVPPPLD QEEMES |
| Purity : |
Purity 97.4% by SDSPAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
