| Description : |
c-Jun N-terminal kinases (JNKs), a stress-activated protein kinase (SAPK), is involved in cellular responses to environmental stresses. JNK kinases can be activated by stimuli such as UV light, radiation, protein synthesis inhibitors, ceramide, DNA-damaging drugs, chemopreventive drugs, TNF-a, and interleukin 1. JNK binds to an NH2-terminal region of ATF2 and c-Jun and phosphorylates two sites within the activation domain of each transcription factor. Three JNK genes (Jnk1, -2 and -3) have been identified in humans; however, splice variants result in a total of 10 isoforms. Therapeutic inhibition of JNK may provide clinical benefit in diseases as diverse as immune inflammatory (joint, bowel, pulmonary), ischemic injury (brain, cardiac) and neurodegenerative (Alzheimer's, Parkinson's) diseases. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
2.453mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Liquid at - 20 centigrade. |
| Bio-activity : |
As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with MKK4, active and MKK7 beta 1, active. |
| Molecular Mass : |
49.2 kDa |
| AA Sequence : |
1 MHHHHHHMSD SKCDSQFYSV QVADSTFTVL KRYQQLKPIG SGAQGIVCAA FDTVLGINVA 61 VKKLSRPFQN QTHAKRAYRE LVLLKCVNHK NIISLLNVFT PQKTLEEFQD VYLVMELMDA 121 NLCQVIHMEL DHERMSYLLY QMLCGIKHLH SAGIIHRDLK PSNIVVKSDC TLKILDFGLA 181 RTACTNFMMT PYVVTRYYRA PEVILGMGYK ENVDIWSVGC IMGELVKGCV IFQGTDHIDQ 241 WNKVIEQLGT PSAEFMKKLQ PTVRNYVENR PKYPGIKFEE LFPDWIFPSE SERDKIKTSQ 301 ARDLLSKMLV IDPDKRISVD EALRHPYITV WYDPAEAEAP PPQIYDAQLE EREHAIEEWK 361 ELIYKEVMDW EERSKNGVVK DQPSDAAVSS NATPSQSSSI NDISSMSTEQ TLASDTDSSL 421 DASTGPLEGC R |
| Purity : |
Purity 99.3% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -20 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
