Unactive Recombinant Human SGK1(S422D)(60-end) Mutation Protein, N-His-tagged
Cat.No. : | SGK1-223H |
Product Overview : | N-terminal 6His-tagged, recombinant, human SGK1, amino acids 60– end, containing the mutation S422D. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose. |
- Specification
- Gene Information
- Related Products
Description : | The serum/glucocorticoid regulated kinases (SGK1,2,3) are serine/threonine kinases that belong to the AGC (protein kinase A; protein kinase G and protein kinase C) subfamily that includes cAMP and cGMP dependent kinases, PKB and PKC. SGK1 (SGK) is predominately expressed in kidney, liver and pancreas. The catalytic domain of serum/glucocorticoid regulated protein kinase is most similar to PKB and activated via phosphorylation mechanism when cells are stimulated with insulin, IGF1 or serum, or exposed to oxidative stress. |
Source : | Sf21 |
Species : | Human |
Tag : | His |
Protein length : | 60-end |
Form : | 2.935mg/ml of enzyme in 50mM Tris/HCl pH7.5, 0.1mM EGTA, 150mM NaCl, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, 270mM sucrose. Frozen solution. |
Bio-activity : | 1451U/mg after activation with PDK1 as described below. One Unit of SGK1 (S422D) activity is defined as 1nmol phosphate incorporated into Crosstide in one minute. |
Molecular Mass : | 45.7 kDa |
AA Sequence : | 1 MSYYHHHHHH DYDIPTTENL YFQGAMGISQ PQEPELMNAN PSPPPSPSQQ INLGPSSNPH 61 AKPSDFHFLK VIGKGSFGKV LLARHKAEEV FYAVKVLQKK AILKKKEEKH IMSERNVLLK 121 NVKHPFLVGL HFSFQTADKL YFVLDYINGG ELFYHLQRER CFLEPRARFY AAEIASALGY 181 LHSLNIVYRD LKPENILLDS QGHIVLTDFG LCKENIEHNS TTSTFCGTPE YLAPEVLHKQ 241 PYDRTVDWWC LGAVLYEMLY GLPPFYSRNT AEMYDNILNK PLQLKPNITN SARHLLEGLL 301 QKDRTKRLGA KDDFMEIKSH VFFSLINWDD LINKKITPPF NPNVSGPNDL RHFDPEFTEE 361 PVPNSIGKSP DSVLVTASVK EAAEAFLGFD YAPPTDSFL |
Purity : | Purity 97.3% by SDS-PAGE and Coomassie blue staining. |
Applications : | Drug Discovery & Development |
Storage : | 6 months at -70 centigrade. |
Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
Gene Name : | SGK1 serum/glucocorticoid regulated kinase 1 [ Homo sapiens (human) ] |
Official Symbol : | SGK1 |
Synonyms : | SGK1; serum/glucocorticoid regulated kinase 1; SGK |
Gene ID : | 6446 |
mRNA Refseq : | NM_005627.4 |
Protein Refseq : | NP_005618.2 |
MIM : | 602958 |
UniProt ID : | O00141 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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