Unactive Recombinant Human SGK2(S416D)(54-end) Mutation Protein, N-His-tagged

• Cat.No.: SGK2-224H
• Product Overview: N-terminal 6His-tagged, recombinant, human SGK2, amino acids 54–end, containing the mutation S416D. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The recombinant protein contains an amino acid substitution S416D with reference to the native sequence. The S416D mutation in the beta isoform is analogous to the S356D substitution in the alpha isoform and S422D in SGK1, both of which are reported to increase activity.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 54-end
• Form: 1.39mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with PDK1.
• Molecular Mass: 45.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGIQL PDHCYRMNSS PAGTPSPQPS RANGNINLGP 61 SANPNAQPTD FDFLKVIGKG NYGKVLLAKR KSDGAFYAVK VLQKKSILKK KEQSHIMAER 121 SVLLKNVRHP FLVGLRYSFQ TPEKLYFVLD YVNGGELFFH LQRERRFLEP RARFYAAEVA 181 SAIGYLHSLN IIYRDLKPEN ILLDCQGHVV LTDFGLCKEG VEPEDTTSTF CGTPEYLAPE 241 VLRKEPYDRA VDWWCLGAVL YEMLHGLPPF YSQDVSQMYE NILHQPLQIP GGRTVAACDL 301 LQSLLHKDQR QRLGSKADFL EIKNHVFFSP INWDDLYHKR LTPPFNPNVT GPADLKHFDP 361 EFTQEAVSKS IGCTPDTVAS SSGASSAFLG FDYAPEDDDI LDC
• Purity: Purity 73% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SGK2 serum/glucocorticoid regulated kinase 2 [ Homo sapiens (human) ]
• Official Symbol: SGK2
• Synonyms: SGK2; serum/glucocorticoid regulated kinase 2; H-SGK2; dJ138B7.2
• Gene ID: 10110
• mRNA Refseq: NM_016276.5
• Protein Refseq: NP_057360.3
• MIM: 607589
• UniProt ID: Q9HBY8