Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Epigenetic Kits > BRD2 bromodomain 2 TR-FRET Assay Kit

BRD2 bromodomain 2 TR-FRET Assay Kit

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GST(7)
His(7)
His & FLAG(1)
N/A(1)
Cat.No.:
Kit-1925
Size:
9600 wells
Description:
Bromodomains recognize acetylated lysine residues and recruit regulatory complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. The isolated individual or tandem bromodomains of BRD2 and BRD4 bind acetylated histone tails, which couples histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of bromodomain interactions hold promise as useful therapeutics for human disease. The BRD2 Bromodomain 2 TR-FRET Assay Kit is a homogeneous, TR-FRET assay method amenable to rapid characterization of inhibitors of bromodomain/acetylated peptide interaction in a high throughput format. This screening assay kit is robust, displaying a Z" >0.6
Storage:
-80°C
Kit Components:
BRD2 bromodomain 2 Europium Chelate: 1 vial/420 wells; 5 vials/420 wells; 5 vials/2,100 wells; -80°CBRD2 bromodomain 2 Ligand/APC Acceptor Mixture: 1 vial/420 wells; 5 vials/420 wells; 5 vials/2,100 wells; -80°CTR-FRET Assay Buffer (10X): 1 vial/2 ml; 1 vial/10 ml; 5 vials/10 ml; -20°CTR-FRET Assay Buffer Additive: 1 vial/200 mg; 1 vial/1 g; 5 vials/1 g; -20°C(+)-JQ1 Positive Control*: 1 vial/8 nmol; 5 vials/8 nmol; 5 vials/40 nmol; -80°C384-Well Solid Plate (low volume; black):1 plate; 5 plates; 25 plates; Room temperatureFoil Plate Covers: 1 cover; 5 covers; 25 covers; Room temperature


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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