Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Assay Kits > Epigenetic Kits > BRG1 bromodomain TR-FRET Assay Kit

BRG1 bromodomain TR-FRET Assay Kit

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Cat.No.:
Kit-1934
Size:
9600 wells
Description:
The SWI/SNF family of ATP-dependent chromatin remodeling enzymes catalyzes structural changes that allow proteins to access the nucleosomal DNA, alter the position of the nucleosomes on the DNA, or eject the histone octamer from the template. Mammalian SWI/SNF remodelers contain either BRM or BRG1 as their catalytic subunit. These subunits also contain a C-terminal bromodomain that binds to acetylated residues on histone tails. Stereotypical inhibitors of BET family bromodomains (JQ-1 and I-BET) do not inhibit the interaction of BRG1 with peptide binding partners (IC50 >100µM). Several subunits of the SWI/SNF complex exhibit tumor suppressor activity and BRG1 is mutated or expression is absent in a significant number of non-small-cell lung cancer samples. The BRG1 bromodomain TR-FRET Assay Kit is a homogeneous, TR-FRET assay method amenable to rapid characterization of inhibitors of bromodomain/acetylated peptide interaction in a high throughput format. This screening assay is robust, displaying a Z >0.7.
Storage:
-80°C
Kit Components:
BRG1 bromodomain Europium Chelate: 1 vial/420 wells; 5 vials/420 wells; 5 vials/2,100 wells; -80°CBRG1 bromodomain Ligand/APC Acceptor Mixture: 1 vial/420 wells; 5 vials/420 wells; 5 vials/ 2,100 wells; -80°CTR-FRET Assay Buffer (10X): 1 vial/2 ml; 1 vial/10 ml; 5 vials/10 ml; -20°CTR-FRET Assay Buffer Additive: 1 vial/200 mg; 1 vial/1 g; 5 vials/1 g; -20°CH3 Positive Control: 1 vial/2.5 nmol; 5 vials/2.5 nmol; 5 vials/12.5 nmol; -80°C384-Well Solid Plate (low volume; black): 1 plate; 5 plates; 25 plates; Room temperatureFoil Plate Covers: 1 cover; 5 covers; 25 covers; Room temperature


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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