Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > HIV-1 Protease Fluorometric Activity Assay Kit

HIV-1 Protease Fluorometric Activity Assay Kit

"Protease" Related Products.
By Species
By Tag
Product Overview:
HIV-1 Activity assay kit utilizes the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release the free fluorophore which can be easily quantified (Ex/Em = 330/450 nm) using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect HIV-1 protease activity in samples and of purified enzyme.
100 assays
Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV1 protease, HIV virions remain non-infectious.
Detect activity of recombinant HIV-1 proteaseDetermine activity of purified HIV-1 protease
Store kit at -80°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read the entire protocol before performing the assay.HIV-1 Protease Assay and Dilution Buffers: Bring to room temperature before use. Store at -20°C.HIV-1 Protease: Add 13 µl of HIV Protease Dilution Buffer to the vial. Aliquot and store at -80°C. Avoid repeated freeze/thaw.
Kit Components:
HIV-1 Protease Assay Buffer: 25 mlHIV-1 Protease Dilution Buffer: 1 mlHIV-1 Protease Substrate: 0.2 mlHIV-1 Protease (Positive Control): 7 μlFl. Standard (10 mM in DMSO): 20 μl
Detection method:
Fluorescence (Ex/Em = 330/450 nm)
Compatible Sample Types:
Features & Benefits:
• Simple, sensitive assay.• Fluorometric detection

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.