Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > P300 (KAT3B) Fluorogenic Assay Kit
"P300" Related Products.
96 reactions
The Fluorogenic p300 Assay Kit is a homogeneous, fluorogenic assay designed to screen for inhibitors of p300 (also known as KAT3B). The Fluorogenic p300 Assay Kit is based on the transfer of an acetyl group from acetyl CoA to a peptide substrate. After incubation with acetyl CoA and the substrate, the P300 enzyme generates acetylated H3 peptide and CoASH. No time-consuming washing steps are required. The thiol groups of CoASH can be detected with fluorogenic reagent at excitation = 360 nm and emission = 460 nm.
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components:
P300 human recombinant enzyme: 10 μg; -80°C Acetyl CoA (1 mM): 100 μl; -20°C 10X HAT assay buffer: 5 ml; -20°C H3 peptide: 500 μl; -20°C Fluorescence Developer: 250 μl; -20°C Developer Solution: 10 ml; -20°C HAT Stop Solution: 5 ml; Room temp. black, low binding NUNC black microtiter plate: 1 plate; Room temp.

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.