"PARP1" Related Products

Expand
Expand

PARP1 Chemiluminescent Assay Kit (384-well)

Cat.No.: Kit-1793
Size: 384 reactions
Description: The PARP1 Chemiluminescent Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: PARP1: 2 x 5 µg; -80°C 5x histone mixture: 2 x 1 ml; -80°C 10x Assay Mixture Containing Biotinylated Substrate: 2 x 300 µl; -80°C 10x PARP assay buffer: 2 x 1 ml; -20°C Blocking buffer: 50 ml; +4°C Activated DNA (5x): 2 x 500 µl; -80°C Streptavidin-HRP: 2 x 100 µl; +4°C HRP chemiluminescent substrate A (translucent bottle): 2 x 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 2 x 6 ml; +4°C 384-well plate: 1; Room Temp.

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.