Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > PDE Activity Colorimetric Assay Kit

PDE Activity Colorimetric Assay Kit

"PDE" Related Products.
Product Overview:
PDE Activity Assay Kit (Colorimetric) combines a special dual enzyme system with Green Assay Reagent for phosphate detection to create a unique, non-radioactive, colorimetric assay to detect phosphodiesterase (PDE) activity. This HTS-friendly, mix and read system may be used to screen inhibitors and modulators of cyclic nucleotide phosphodiesterase activity. 96-well microplate format permits rapid assays of large numbers of samples. The basis for the assay is the cleavage of cAMP or cGMP by a cyclic nucleotide phosphodiesterase. The kit includes a Type I PDE as positive control and a non-specific PDE inhibitor, 2-isobutyl-1-methylzanthine (IBMX) as test control for inhibitor screening.
1 x 96 tests
Functional Studies more details
Kit Components:
Components: 1 x 96 tests; 3,5-cAMP Substrate: 1 x 2ml; 3,5-cGMP Substrate: 1 x 2ml; 5 amp standard: 1 x 1ml; 5-GMP Standard: 1 x 1ml; 5-Nucleotidase (from Crotalus atrox venom): 1 x 1ml; 96-well Clear Microplate (1/2 Volume): 1 unit; Desalting Column: 1 unit; Desalting Resin: 1 x 1g; Green Assay Reagent: 1 x 20ml; PDE Assay Buffer: 1 x 40ml; PDE Enzyme (from bovine brain): 5 vials; PDE Inhibitor: 1 x 200µl
Detection method:
Compatible Sample Types:
Tissue, Inhibitor compounds

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2016 Creative Biomart. All Rights Reserved.