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Home > Products > Assay Kits > Activity Kits > Protease Activity Fluorometric Assay Kit

Protease Activity Fluorometric Assay Kit

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Product Overview:
Simple procedure; takes ~60 minutes Fast and convenient Sensitive assay for measuring protease activity in a number of sample types
100 assays
Proteases are naturally present in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades. Proteases can either break specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein"s function), an activation of a function (preform to mature form) or it can be a signal in a signaling pathway. BioVision"s Protease Activity Assay Kit is designed for the quantitative determination of proteases present in the protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-Casein is highly quenched. Upon digestion by proteases present in the sample the FITC-Casein substrate is cleaved into smaller peptides which abolishes the quenching of the fluorescence label. The fluorescence of the FITC-labeled peptide fragments is measured at Ex/Em = 485/530 nm. The kit is supplied with our Mass Spectrometry Grade (MSG), chemically stabilized Trypsin for use as a general protease control. However, other protease standard controls can also be used. This kit is easy to use and can detect< 500 pg/well Trypsin present in the sample
This kit can detect< 500 pg/well Trypsin present in the sample
For Research Use Only! Not For Use in Humans
Kit Components:
• Assay Buffer • Substrate (lyophilized) • FITC Standard (25 μM) • Positive Control (lyophilized)
Detection method:
Fluorescence (Ex/Em 485/530 nm)
Compatible Sample Types:
Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food samples, etc.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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