Recombinant Chicken IL17A protein, His & GST-tagged
Cat.No. : | IL17A-869C |
Product Overview : | Recombinant Chicken IL17A aa. (Ile25~Ala169) fused with N-terminal His & GST tag was produced in E. coli cells. |
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Source : | E. coli |
Species : | Chicken |
Tag : | His & GST |
Form : | Freeze-dried powder |
Molecular Mass : | 46kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Ile25~Ala169 |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method) |
Purity : | > 95% |
Characteristic : | The isoelectric point is 7. |
Applications : | SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : | 200μg/mL |
Storage buffer : | PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300 |
Reconstitution : | Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name : | IL17A interleukin 17A [ Gallus gallus (chicken) ] |
Official Symbol : | IL17A |
Synonyms : | IL17A; interleukin 17A; IL17; IL-17; IL-17F; ChIL-17; interleukin-17A; interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin-17 |
Gene ID : | 395111 |
mRNA Refseq : | NM_204460.1 |
Protein Refseq : | NP_989791.1 |
UniProt ID : | Q7T1P7 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAnimal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.
Inducing IL17A expression in T cells involves culturing them with specific cytokines.
Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.
Neutralizing antibodies block IL17A's effects in cell cultures or animal models.
Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.
IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.
Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.
Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.
Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.
Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.
Customer Reviews (3)
Write a reviewGood for receptor studies.
Effective in Th17 differentiation.
High bioactivity in various tissue types.
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