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Recombinant Human ADAM28 protein, His-tagged

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Product Overview:
Recombinant Human ADAM28(Leu72 & Leu191-Ala623) fused with His tag at C-terminal was expressed in CHO.
A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway. After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 aa extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain. Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains. Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28. ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis, and exhibits catalytic activity to insulin-like growth factor binding protein-3. Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction. ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4b1(9). R&D Systems in-house testing also indicates that ADAM28 cleaves IGFBP-3.
Tag :
Predicted N Terminal:
Leu72 & Leu191
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.2-1 μg/mL.
Molecular Mass:
Predicted Molecular Mass: 49 & 62 kDa
SDS-PAGE: 59-80 kDa, reducing conditions
<0.1 EU per 1 μg of the protein by the LAL method.
>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 200 μg/mL in water.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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