Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human BMP4 protein, His & T7-tagged

Recombinant Human BMP4 protein, His & T7-tagged

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Cat.No.:
BMP4-569H
Product Overview:
Recombinant Human BMP4 (P12644) aa. (Ser22~Arg408) fused with N-terminal His & T7 tag was produced in E. coli cells.
Description:
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers.
Source:
E. coli
Species:
Human
Tag :
His & T7
Form:
Freeze-dried powder
Molecular Mass:
Predicted Molecular Mass: 48.2kDa
Protein length:
Ser22~Arg408 (Accession # P12644
Endotoxin:
<1.0EU per 1µg (determined by the LAL method)
Purity:
> 95%
Characteristic:
The isoelectric point is 9.
Applications:
SDS-PAGE; WB; ELISA; IP.
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage:
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer:
PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution:
Reconstitute in sterile PBS, pH7.2-pH7.4


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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