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Home > Products > Recombinant Proteins > Recombinant Human COL2A1 protein, His-tagged

Recombinant Human COL2A1 protein, His-tagged

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Cat.No.:
COL2A1-26H
Product Overview:
Recombinant Human COL2A1(Gln26-Arg266 & Gly957-Leu1487) fused with His tag at N-terminal was expressed in CHO.
Description:
Type II collagen is an essential component of cartilage. It is essential for skeletal development and for the ability of cartilage to resist compressive forces. Type II collagen is a homotrimer of a1(II) chains. Defects in Type II collagen are the cause of numerous diseases, including chondrodysplasias and arthritis. This recombinant mini pro-a1(II) collagen consists of the N‑terminal propeptide, 108 Gly-X-Y repeats, and the C‑terminal propeptide. Proteolytic processing of the C‑terminal propeptide by the procollagen C-proteinase (BMP-1) releases chondrocalcin, a calcium binding protein.
Source:
CHO
Species:
Human
Tag:
His
Predicted N Terminal:
His & Asp1242
Form:
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Bio-activity:
Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP‑1/PCP. >50% of full-length Pro-Collagen II is cleaved by Recombinant Human BMP‑1/PCP.
Molecular Mass:
Predicted Molecular Mass: 78 kDa;SDS-PAGE: 92-100 kDa, 65-70 kDa, 36 kDa, reducing conditions.
Endotoxin:
<1.0 EU per 1 μg of the protein by the LAL method.
Purity:
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Storage:
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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