Recombinant human CSF1 (NP_757349.1) (Glu33-Asn190) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured in a cell proliferation assay using M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1-4 ng/mL.
The recombinant human CSF1 consists 168 amino acids and predicts a molecular mass of 19.8 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>(45.8+27.8+25.7) % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.