Recombinant Human IL17RB Protein, Fc-tagged
Recombinant human IL17BR (NP_061195.2) (Met1-Gly289) was expressed with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its binding ability in a functional ELISA.2. Immobilized human IL17BR-Fc at 10μg/mL (100μL/well) can bind biotinylated human Fc-IL25 (Cat:10096-H01H).The EC50 of human Fc-IL25 (Cat:10096-H01H) is 4.5-10.4ng/mL.
The recombinant human IL17BR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 513 amino acids and has a predicted molecular mass of 56.9 kDa. The apparent molecular mass of the protein is approximately 67 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>85 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.