Recombinant Human Activin beta A(Gly311 - Ser426)/Human Activin beta B(Gly293 - Ala407) was coexpressed in CHO.
Predicted N Terminal:
Gly311( beta A subunit) & Gly293 ( beta B subunit)
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is typically 0.2‑1.2 ng/mL.
Predicted Molecular Mass: 12.8 ( beta A subunit) and 13 ( beta B subunit) kDa;SDS-PAGE: 14 kDa, reducing conditions.
<0.1 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.