Recombinant Human MMP9(Ala20-Asp707) fused with a C-terminal 8 His-tag and a linker sequence was expressed in HEK293.
Predicted N Terminal:
0.22 µm filtered protein solution is in TCN (25 mM TRIS, 10 mM CaCl2, 150 mM NaCl, pH 7.5).
Human MMP-9 cleaves the peptide substrate Mca-PLGL-Dpa-AR-NH2 with an activity above 1000 pmol/min/µg.
This 722 amino acid recombinant protein has a predicted molecular mass of approximately 80 kD. The protein migrates at about 92 kD in DTT-reducing conditions and 250 kD in non-reducing conditions by SDS-PAGE.
Less than 1.0 EU per µg of protein as determine by the LAL method.
>95%, as determined by Coomassie stained SDS-PAGE.
Unopened vial can be stored at -70 centigrade for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.