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Recombinant Human MMP9 protein, His-tagged

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GST(1)
His(19)
N/A(5)
None(2)
T7(1)
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Cat.No.:
MMP9-4893H
Product Overview:
Recombinant Human MMP9(Ala20-Asp707) fused with a C-terminal 8 His-tag and a linker sequence was expressed in HEK293.
Source:
HEK293
Species:
Human
Tag:
His
Predicted N Terminal:
Ala
Form:
0.22 µm filtered protein solution is in TCN (25 mM TRIS, 10 mM CaCl2, 150 mM NaCl, pH 7.5).
Bio-activity:
Human MMP-9 cleaves the peptide substrate Mca-PLGL-Dpa-AR-NH2 with an activity above 1000 pmol/min/µg.
Molecular Mass:
This 722 amino acid recombinant protein has a predicted molecular mass of approximately 80 kD. The protein migrates at about 92 kD in DTT-reducing conditions and 250 kD in non-reducing conditions by SDS-PAGE.
Endotoxin:
Less than 1.0 EU per µg of protein as determine by the LAL method.
Purity:
>95%, as determined by Coomassie stained SDS-PAGE.
Storage:
Unopened vial can be stored at -70 centigrade for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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