The His-tag biotinylated recombinant protein is purified by affinity chromatography in combination with FPLC columns. Then His-tag was removed by protease.
Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family. SRC1 has been shown to be over expressed in some cancers. SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction. The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions. This protein does not contain His tag and will be useful for assays where a native untagged protein is desired. Lane 1 corresponds to 6His-tagged and biotinylated SRC-RID; Lane 2 corresponds to biotinylated SRC-RID without 6His tag.
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTERHKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDHQLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT
Purified biotinylated SRC1-RID is greater than 90% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
The biotinylated SCR1-RID (627-786) can be used for protein-protein interaction assays, particularly suitable for high throughput screening (HTS) assays.
Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.