Recombinant Human NTHL1 fused with MYC/DDK tag at C-terminal was expressed in HEK293.
The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity.
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
> 80% as determined by SDS-PAGE and Coomassie blue staining
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
>50 ug/mL as determined by microplate BCA method