Recombinant Human PCMT1(2-end) fused with GST tag at N-terminal was expressed in Insect cells.
PCMT1 is a member of the L-isoaspartyl/D-aspartyl protein methyltransferase family, which plays an important role in protein repair. Downregulation of PCMT1 leads to hyperactivation of MAPK pathway upon EGF stimulation. PCMT1 is regulated by cell-matrix interactions, and linked to various biological processes including wound healing, cell migration, and tumor metastasis.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
1 year at -70 centigrade from the date of shipment
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.