Recombinant Human SENP2 fused with His tag at N-terminal was expressed in E. coli.
SUMO1 (UBL1; MIM 601912) is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes that process newly synthesized SUMO1 into the conjugatable form and catalyze the deconjugation of SUMO1-containing species.
50 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol
≥95% by SDS-PAGE and RP-HPLC
1 Unit of SUMO Protease 2 cleaves >90% of 100 µg SUMO Protease 2 Control Protein in 1 hour (37 centigrade)
Useful for removal of SUMO-tags from recombinant SUMO fusion proteins
Store at or below –80 centigrade. Stable as supplied for up to 1 year when stored dessicated at –80 centigrade. Avoid freeze/thaw cycles.