Recombinant Human cytomegalovirus GB(Val 23 - Lys 700) fused with a human IgG1 Fc tag at the C-terminus, with furin cleavage site mutated from 'RTKR' to 'TTQT' was expressed in HEK293.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 104 kDa. The predicted N-terminus is Val 23. DTT-reduced Protein migrates as 140-150 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh CMV-GB Fc Chimera by the LAL method.
>85% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).