Recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA. 1. Immobilized Spike (HCoV-EMC/2012) (ECD, aa 1-1297) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc. The EC50 of biotinylated human ACE2-Fc is 0.9-1.4 μg/ml. 2. Immobilized Spike (HCoV
The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289 amino acids and has a predicted molecular mass of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>85 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.