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Recombinant MERS-CoV (NCoV / Novel coronavirus) S Protein, His-tagged

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Product Name:
Recombinant MERS-CoV (NCoV / Novel coronavirus) S Protein, His-tagged
Product Overview:
Recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012)( AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus.
Baculovirus-Insect Cells
Predicted N Terminal:
Tyr 18
Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA. 1. Immobilized Spike (HCoV-EMC/2012) (ECD, aa 1-1297) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc. The EC50 of biotinylated human ACE2-Fc is 0.9-1.4 μg/ml. 2. Immobilized Spike (HCoV
Molecular Mass:
The recombinant extracellular domain of spike protein (Human betacoronavirus 2c EMC/2012) comprises 1289 amino acids and has a predicted molecular mass of 142.2 kDa. It migrates as an approximately 157 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>85 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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