Recombinant Mouse Gpc1(Asp24-Ser529) fused with His tag at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. Immobilized rmGlypican 1 at 3 µg/mL (100 µL/well) will bind rhFGF-basic with an apparent KD < 1 nM.
Predicted Molecular Mass: 56.4 kDa;SDS-PAGE: 57-62 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 500 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.