Recombinant Mouse IFNG Protein, His-tagged
Recombinant mouse IFNG (P01580) (Met 1-Cys 155) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNG-His at 10 μg/ml (100 μl/wel
The recombinant mouse IFNG comprises 144 amino acids and has a calculated molecular mass of 17 kDa. As a result of glycosylation, the apparent molecular mass of rn IFNG is approximately 21 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>75 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.