Active Recombinant Mouse IFNG Protein, His-tagged

• Cat.No.: IFNG-893M
• Product Overview: Recombinant mouse IFNG (P01580) (Met 1-Cys 155) was expressed, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: His 23
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNG-His at 10 μg/ml (100 μl/wel
• Molecular Mass: The recombinant mouse IFNG comprises 144 amino acids and has a calculated molecular mass of 17 kDa. As a result of glycosylation, the apparent molecular mass of rn IFNG is approximately 21 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >75 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Ifng interferon gamma [ Mus musculus ]
• Official Symbol: IFNG
• Synonyms: IFNG; interferon gamma; IFN-gamma; gamma interferon; Ifg; IFN-g
• Gene ID: 15978
• mRNA Refseq: NM_008337
• Protein Refseq: NP_032363
• Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved
• Function: cytokine activity; interferon-gamma receptor binding