| Description : |
Sharing 41% sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ)is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ–induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. |
| Source : |
E. coli |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50< 0.15 ng/mL, measured by cytotoxicity assay using WEHI-279 cells. |
| Molecular Mass : |
15 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
134 |
| AA Sequence : |
MHGTVIESLESLNNYFNSSGIDVEE KSLFLDIWRNWQKDGDMKILQSQII SFYLRLFEVLKDNQAISNNISVIES HLITTFFSNSKAKKDAFMSIAKFEV NNPQVQRQAFNELIRVVHQLLPESS LRKRKRSRC |
| Endotoxin : |
< 1 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by reducing SDS-PAGE. |
| Storage : |
Lyophilized recombinant mouse IFN gamma (rmIFN-γ) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rmIFN-γ should be stable up to 1week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
