"Il21" Related Products

Recombinant Mouse Il21 Protein

Cat.No.: Il21-844M
Product Overview: Recombinant soluble form of mouse IL21 (Q9ES17) (Pro 25-Ser 146) was expressed, with an initial Met at the C-terminus.
Source: E. coli
Species: Mouse
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.51, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 4-20 ng/ml.
Molecular Mass: The recombinant mouse IL21 consists of 123 amino acids and has a calculated molecular mass of 14.3KDa. It migrates as a 15 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Il21 interleukin 21 [ Mus musculus ]
Official Symbol: Il21
Synonyms: IL21; interleukin 21; interleukin-21; IL-21;
Gene ID: 60505
mRNA Refseq: NM_021782
Protein Refseq: NP_068554
MIM:
UniProt ID:
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function: cytokine activity; cytokine receptor binding; interleukin-2 receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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