Acitve Recombinant Marmoset IL12B protein(Met1-Asn328), hFc-tagged

• Cat.No.: IL12B-1381M
• Product Overview: Recombinant Marmoset IL12B (F7IQ84) (Met1-Asn328) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Marmoset
• Tag: C-hFc
• Protein length: Met1-Asn328
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2.Immobilized marmoset IL12B-Fc at 10 μg/mL (100 μL/well) can bind biotinylated Human IL12RB1-His. The EC50 of Human IL12RB1-His is 0.66-1.53 μg/mL.
• Molecular Mass: The recombinant marmoset IL12B is a disulfide-linked homodimer. The reduced monomer comprises 547 amino acids and has a calculated molecular mass of 61.9 KDa.The apparent molecular mass of the protein is approximately 66 KDa in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Official Symbol: IL12B

Gene Information

• Official Symbol: IL12B