Active GMP Recombinant Human IL12 protein
Cat.No. : | IL12-159HG |
Product Overview : | GMP Recombinant Human IL12 protein(NP_002178.2)(210 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines. |
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Source : | HEK293 |
Species : | Human |
Bio-activity : | >1.0×10^7 IU/mg |
Molecular Mass : | Recombinant human p30 subunit contains 210 aa, recombinant human p40 subunit contains 319 aa. It has a predicted MW of 60 kDa. |
Protein length : | DNA sequence encoding Human p35 Subunit(UniProtKB/Swiss-Prot: P29459.2),p40 Subunit (NP_002178.2) was expressed with His tag at the C-terminal. |
Endotoxin : | 0.01-0.02EU/ug |
Purity : | >=95% by SDS-PAGE and HPLC |
Storage : | The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | IL12B interleukin 12B [ Homo sapiens (human) ] |
Official Symbol : | IL12B |
Synonyms : | CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B |
Gene ID : | 3593 |
mRNA Refseq : | NM_002187.3 |
Protein Refseq : | NP_002178.2 |
MIM : | 161561 |
UniProt ID : | P29460 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionFluorescence microscopy tracks IL12 internalization and trafficking.
Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.
Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.
Reporter gene assays analyze IL12's promoter activity and response elements.
Super-resolution imaging details IL12's cellular distribution and dynamics.
3D cell culture models reveal IL12's effects on tissue microenvironments.
Co-localization studies and co-IP assays identify IL12's binding partners.
Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.
ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.
Transgenic models elucidate IL12's roles in immune response regulation.
Customer Reviews (3)
Write a reviewEffective in Th1 cell differentiation.
High specificity in ELISA.
Stable under storage.
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