Active GMP Recombinant Human IL12 Protein

• Cat.No.: IL12-160HG
• Product Overview: Recombinant p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; Recombinant p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified in an animal component free process under cGMP guidelines.

Specification

• Source: HEK293
• Species: Human
• Predicted N Terminal: Arg 23 & Ile 23
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween80.
• Bio-activity: Measured in a cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically 0.4-2 ng/ml. The specific activity of recombinant human IL-12 is approximately 1.3 x 10^4 units/μg, which is calibrated against recombinant human IL-12 WHO Standard (NIBSC code: 95/544).
• Molecular Mass: The GMP recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa.
• Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ]
• Official Symbol: IL12A
• Synonyms: IL12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); NKSF1; interleukin-12 subunit alpha; CLMF; cytotoxic lymphocyte maturation factor 1; p35; IL 12; subunit p35; IL 12A; IL35 subunit; interleukin 12; interleukin 12 alpha chain; natural killer cell stimulatory factor 1; 35 kD subunit; NF cell stimulatory factor chain 1; NFSK; CLMF p35; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, 35 kD subunit; P35; CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF; NKSF2; P35
• Gene ID: 3592
• mRNA Refseq: NM_000882
• Protein Refseq: NP_000873
• MIM: 161560
• UniProt ID: P29459

Reviews

01/07/2022

Effective in Th1 cell differentiation.

04/09/2020

High specificity in ELISA.

08/02/2018

Stable under storage.

Q&As

How does fluorescence microscopy track IL12 internalization and intracellular trafficking?

Fluorescence microscopy tracks IL12 internalization and trafficking.

What techniques dissect IL12's signaling cascades and crosstalk with other cytokines?

Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

What strategies assess IL12's polarization of T cell subsets and cytokine profiles?

Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

How are reporter gene assays tailored to study IL12's promoter activity and response elements?

Reporter gene assays analyze IL12's promoter activity and response elements.

What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide?

Super-resolution imaging details IL12's cellular distribution and dynamics.

What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments?

3D cell culture models reveal IL12's effects on tissue microenvironments.

How do protein-protein interaction assays decipher IL12's binding partners in cellular context?

Co-localization studies and co-IP assays identify IL12's binding partners.

What methods elucidate IL12's receptor complex formation and stoichiometry?

Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks?

ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

Could you explain how transgenic models help dissect IL12's roles in immune response modulation?

Transgenic models elucidate IL12's roles in immune response regulation.