Active GMP Recombinant Human IL12 Protein

• Cat.No.: IL12-01HG
• Product Overview: Recombinant human IL12 protein, was expressed in CHO-K1 cell-derived in an animal component free process under cGMP guidelines.

Specification

• Description: Interleukin-12 (IL-12) is a cytokine with a wide range of biological activities and is mainly produced by activated inflammatory cells. However, it mainly acts on lymphocytes. IL-12 is a determinant of the Th1 cellular immune response and effectively promotes the production of Th1-like cytokines such as IFN-γ, and on the other hand inhibits the production of Th2-like factors such as IL-4 and IL-13, thereby suppressing the Th2-type response. reduction or absence of IL-12 results in a Th2-dominant immune response. In addition, IL-12 induces the production of a variety of cytokines, such as IFN-γ and IL-10. IL-12 is the most potent cytokine that induces the production of IFN-γ by T lymphocytes, especially activated T cells and NK cells, and many of the in vivo effects of IL-12 are exerted through the induction of IFN-γ. The biological activity of IL-12 is exerted through the production of IFN-γ by T cells and IL-12 receptors on NK cells are mediated.
• Source: CHO-K1
• Species: Human
• Form: Lyophilized powder
• Endotoxin: <20 EU/mg
• Bio-activity: >1.0 x 10^6 IU/mg
• Purity: >95.0% by high performance liquid chromatography (SEC-HPLC) and SDS-PAGE.
• Storage Buffer: 10mM Phosphate-Buffered Saline (pH 7.2)
• Storage: Stable storage at 4℃ for 7-10 days; for long-term storage, please store at -20℃ after dispensing.
• Sterility: Sterility
• Instructions: It is recommended that lyophilized GMP rhIL12 be dissolved in water for injection or sterilized ultrapure water at a concentration of not less than 100 μg/ml, pending further dilution to working concentration.

Reviews

01/07/2022

Effective in Th1 cell differentiation.

04/09/2020

High specificity in ELISA.

08/02/2018

Stable under storage.

Q&As

How does fluorescence microscopy track IL12 internalization and intracellular trafficking?

Fluorescence microscopy tracks IL12 internalization and trafficking.

What techniques dissect IL12's signaling cascades and crosstalk with other cytokines?

Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

What strategies assess IL12's polarization of T cell subsets and cytokine profiles?

Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

How are reporter gene assays tailored to study IL12's promoter activity and response elements?

Reporter gene assays analyze IL12's promoter activity and response elements.

What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide?

Super-resolution imaging details IL12's cellular distribution and dynamics.

What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments?

3D cell culture models reveal IL12's effects on tissue microenvironments.

How do protein-protein interaction assays decipher IL12's binding partners in cellular context?

Co-localization studies and co-IP assays identify IL12's binding partners.

What methods elucidate IL12's receptor complex formation and stoichiometry?

Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks?

ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

Could you explain how transgenic models help dissect IL12's roles in immune response modulation?

Transgenic models elucidate IL12's roles in immune response regulation.