| Description : |
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by reproductive tract. |
| Source : |
Escherichia coli |
| Species : |
Porcine |
| Tag : |
His |
| Form : |
Lyophilized |
| Bio-activity : |
Measure by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is <3 ng/mL. |
| AA Sequence : |
APTRPPSPVTRPWQHVDAIKEALSL LNNSNDTAAVMNETVDVVCEMFDPQ EPTCVQTRLNLYKQGLRGSLTRLKS PLTLLAKHYEQHCPLTEETSCETQS ITFKSFKDSLNKFLFTIPFDCWGPV KK with polyhistidine tag at the N-terminus |
| Endotoxin : |
<0.1 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>98% as determined by SDS-PAGE. Ni-NTA chromatography |
| Notes : |
Please use within one month after protein reconstitution. |
| Storage : |
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
| Storage Buffer : |
The protein was lyophilized from a solution containing 1X PBS, pH 7.4. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
