| Description : |
High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA-binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by directly contacting with distinct cellular proteins . HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release. |
| Source : |
Calf Thymus |
| Species : |
Bovine |
| Tag : |
N/A |
| Form : |
20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA |
| Bio-activity : |
1 unit equals 1 ng of purified protein. 10-100 units are required for a gel mobility shift assay in a 20μl reaction to super-shift TBP-DNA complex, 50-500 units are required for a reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay. |
| AA Sequence : |
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEEEEE DDDDE |
| Purity : |
>95% as determined by SDS-PAGE |
| Storage : |
-80 °C |
| Concentration : |
900 μg/ml |
| Shipping : |
Dry Ice |
