Active Recombinant Human HMGB1 Protein (Met1-Asp169, C23A, C45A, C106A), N-His-SUMO tagged, Animal-free, Carrier-free

• Cat.No.: HMGB1-144H
• Product Overview: Recombinant Human HMGB1 Protein (Met1-Asp169, C23A, C45A, C106A) with N-His-SUMO tag was expressed in HEK293 and Animal-free as well as Carrier-free.

Specification

• Description: High Mobility Group protein B1 protein (HMGB1) is the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 25 kDa single chain polypeptide containing three domains: two N-terminal HMG boxes A and B, and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu. Post-translational modification on HMGB1 have been reported, affect its localization, receptor interactions, and function. HMGB1, with a disulfide bond between C23 and C45 , have been reported that cause cytokine production and the activation of NF-κB. Otherwise, the fully oxidized form has no immune function, losing its proinflammatory effect and the apoptotic cell death activation function. Here, we developed HMGB1 C23A, C45A & 106A mutant proteins, the fully oxidized HMGB1, eliminant the disulfide bond formation.
• Source: HEK293 cell
• Species: Human
• Tag: N-His-SUMO
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce TNF alpha in RAW264.7 cells. The ED50 for this effect is < 10 μg/mL.
• Molecular Mass: The protein has a calculated MW of 36.33 kDa.
  The protein migrates as 35-48 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Met1-Asp169
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: HMGB1 high mobility group box 1 [ Homo sapiens (human) ]
• Official Symbol: HMGB1
• Synonyms: HMGB1; high mobility group box 1; high mobility group (nonhistone chromosomal) protein 1 , high mobility group box 1 , HMG1; high mobility group protein B1; Amphoterin; DKFZp686A04236; high mobility group protein 1; HMG3; SBP 1; Sulfoglucuronyl carbohydrate binding protein; HMG-1; high-mobility group box 1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; SBP-1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 163905
• UniProt ID: P09429