Active Recombinant Canine IL17A protein, His-tagged

• Cat.No.: IL17A-1588C
• Product Overview: Recombinant Canine IL17A protein(Gly26-Ala155), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Canine
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Monoclonal Anti-Human IL17A Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Canine IL-17A, His Tag with a linear range of 5-39 ng/mL.Immobilized Canine IL-17A, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag with a linear range of 4-63 ng/mL.Immobilized Canine IL-17A, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (MALS verified) with a linear range of 0.1-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 17.0 kDa. The protein migrates as 18 kDa and 22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gly26-Ala155
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.