Active Recombinant Cynomolgus GPC3 protein, Fc-tagged

• Cat.No.: GPC3-1517C
• Product Overview: Recombinant Cynomolgus GPC3 protein(Gln25-His559), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5.
• Bio-activity: Immobilized Human FGF basic, premium grade at 2 μg/mL (100 μL/well) can bind Cynomolgus Glypican 3, Fc Tag with a linear range of 1-31 ng/mL.Immobilized Cynomolgus Glypican 3, Fc Tag at 0.5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPC3 Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.
  This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 49.4 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln25-His559
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.