Active Recombinant Cynomolgus / Rhesus macaque IL15 protein, His-tagged
Cat.No. : | IL15-0177C |
Product Overview : | Recombinant Cynomolgus / Rhesus macaque IL15 protein(Asn49-Ser162), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Cynomolgus / Rhesus macaque |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human IL-15 R alpha, Fc Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-15, His Tag with a linear range of 1-8 ng/mL.Immobilized Anti-IL-15 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-15, His Tag with a linear range of 2-16 ng/mL.Immobilized Cynomolgus / Rhesus macaque IL-15, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) with a linear range of 0.039-0.625 μg/mL.Immobilized Cynomolgus / Rhesus macaque IL-15, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag with a linear range of 0.078-1.25 μg/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 14.9 kDa. The protein migrates as 14 kDa and 16-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : | Asn49-Ser162 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Official Symbol : | IL15 |
Official Symbol : | IL15 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTechniques discern IL15 and IL2 receptor binding kinetics.
Methods explore IL15's role in autoimmune disease mechanisms.
IL15 knockout models provide insights into its diverse functions.
IL15's influence on memory T cell differentiation investigated.
Immunofluorescence microscopy visualizes IL15 subcellular localization.
Approaches validate IL15's involvement in inflammatory responses.
IL15's role in NK cell cytotoxicity analyzed using assays.
ELISA measures IL15 secretion accurately.
qPCR quantifies IL15's impact on regulatory T cells.
Flow cytometry quantifies IL15-driven T-cell proliferation.
Customer Reviews (3)
Write a reviewMapping protein- interactions in DNA transcription for gene regulation.
Suitable for cytotoxicity assays.
Effective in NK cell activation.
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