Active Recombinant Cynomolgus / Rhesus macaque TIGIT protein, mFc-tagged

• Cat.No.: TIGIT-0798C
• Product Overview: Recombinant Cynomolgus / Rhesus macaque TIGIT protein(Met89-Ile208), fused with mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus / Rhesus macaque
• Tag: mFc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rhesus macaque CD155, His Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque TIGIT, Mouse IgG2a Fc Tag with a linear range of 0.3-20 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 40.2 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Met89-Ile208
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.