| Description : |
Interleukin-4 (IL-4) induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B-cell class switching to IgE, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies. |
| Source : |
Yeast |
| Species : |
Equine |
| Form : |
Lyophilized without carrier protein. |
| Bio-activity : |
The biological activity of recombinant equine IL-4 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 40-60 ng/mL. |
| Molecular Mass : |
Equine IL-4 has a predicted molecular weight of 12.8 kDa. |
| AA Sequence : |
GCKYDITLQE IIKTLNNLTD GKGKNSCMEL TVADAFAGPK NTDGKEICRA AKVLQQLYKR HDRSLIKECL SGLDRNLKGM ANGTCCTVNE AKKSTLKDFL ERLKTIMKEK YSKC (114) |
| Purity : |
>95% as visualized by SDS-PAGE analysis. |
| Applications : |
The Equine IL-4 protein can be used in cell culture, as an IL-4 ELISA Standard, and as a Western Blot Control. |
| Storage : |
Stable for up to twelve months from date of receipt at -20oC. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. |
