Active Recombinant Full Length Human RNASE1 Protein, C-Flag-tagged

• Cat.No.: RNASE1-02HFL
• Product Overview: Recombinant Full Length Human RNASE1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme activity
  In vivo treatment
  Cell treatment
  Binding assay
  In vivo treatment
• Molecular Mass: 14.5 kDa
• AA Sequence: MALEKSLVRLLLLVLILLVLGWVQP SLGKESRAKKFQRQHMDSDSSPSSS STYCNQMMRRRNMTQGRCKP VNTFVHEPLVDVQNVCFQEKVTCKN GQGNCYKSNSSMHITDCRLTNGSRY PNCAYRTSPKERHIIVACEG
  SPYVPVHFDASVEDSTTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Secreted Protein, Transmembrane

Gene Information

• Gene Name: RNASE1 ribonuclease A family member 1, pancreatic [ Homo sapiens (human) ]
• Official Symbol: RNASE1
• Synonyms: RAC1; RIB1; RNS1
• Gene ID: 6035
• mRNA Refseq: NM_198235.3
• Protein Refseq: NP_937878.1
• MIM: 180440
• UniProt ID: P07998

Reviews

10/27/2022

RNASE1 have strong anti-contamination ability and are not easily contaminated, which ensures the quality of the product.

07/06/2021

This stability of RNASE1 is impressive, maintaining stable performance regardless of the environment, demonstrating excellent quality and reliability.

01/08/2020

The catalytic activity of RNASE1 is excellent, which can simplify complex chemical reactions and improve yields, and has high scientific research value.

Q&As

Is there an association between RNASE1 protein and insulin secretion?

There is a correlation between RNASE1 protein and insulin secretion. Studies have shown that RNASE1 protein can regulate insulin synthesis and secretion by affecting the internal environment of pancreatic islets.

Is there a regulatory mechanism for RNASE1 proteins?

At present, the regulatory mechanism of RNASE1 protein is poorly understood. However, studies have found that some factors such as glucagon and insulin may be involved in regulating the expression and activity of RNASE1 protein.

Does RNASE1 protein have anti-tumor effects?

The RNASE1 protein is thought to have potential for anti-tumor effects. Studies have shown that it can induce apoptosis of tumor cells and inhibit the proliferation and invasion ability of tumor cells.

What are the applications of RNASE1 protein in clinical diagnosis?

RNASE1 protein has certain application potential in clinical diagnosis. Its hydrolyzed RNA properties can be used to measure the content and stability of RNA, and then evaluate the condition and treatment effect of certain diseases.

What role does RNASE1 protein play in RNA degradation?

The RNASE1 protein participates in the RNA degradation process in the digestive system by cutting the phosphodiester bond of the RNA molecule and degrading it into shorter RNA fragments.

Is RNASE1 protein also expressed in the gut?

The main expression and secretion site of RNASE1 protein is the pancreas, while the expression in the gut is relatively low. But in some cases, other nucleases in the gut may perform a similar function.