Active Recombinant Full Length Human TPX2 Protein, C-Flag-tagged

• Cat.No.: TPX2-222HFL
• Product Overview: Recombinant Full Length Human TPX2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: TPX2 activity in a microtubule binding assay: SAF-A binds to MT in a TPX2-dependent manner. MT binding analysis of isolated SAF-A protein in the presence of different amounts of recombinant TPX2 protein. The lower panel shows western blotting with anti-SAF-A antibody. SAF-A co-pelleted with MT was increased, dependent on the amount of TPX2.
• Molecular Mass: 85.5 kDa
• AA Sequence: MSQVKSSYSYDAPSDFINFSSLDDE GDTQNIDSWFEEKANLENKLLGKNG TGGLFQGKTPLRKANLQQAI VTPLKPVDNTYYKEAEKENLVEQSI PSNACSSLEVEAAISRKTPAQPQRR SLRLSAQKDLEQKEKHHVKM KAKRCATPVIIDEILPSKKMKVSNN KKKPEEEGSAHQDTAEKNASSPEKA KGRHTVPCMPPAKQKFLKST EEQELEKSMKMQQEVVEMRKKNEEF KKLALAGIGQPVKKSVSQVTKSVDF HFRTDERIKQHPKNQEEYKE VNFTSELRKHPSSPARVTKGCTIVK PFNLSQGKKRTFDETVSTYVPLAQQ VEDFHKRTPNRYHLRSKKDD INLLPSKSSVTKICRDPQTPVLQTK HRARAVTCKSTAELEAEELEKLQQY KFKARELDPRILEGGPILPK KPPVKPPTEPIGFDLEIEKRIQERE SKKKTEDEHFEFHSRPCPTKILEDV VGVPEKKVLPITVPKSPAFA LKNRIRMPTKEDEEEDEPVVIKAQP VPHYGVPFKPQIPEARTVEICPFSF DSRDKERQLQKEKKIKELQK GEVPKFKALPLPHFDTINLPEKKVK NVTQIEPFCLETDRRGALKAQTWKH QLEEELRQQKEAACFKARPN TVISQEPFVPKKEKKSVAEGLSGSL VQEPFQLATEKRAKERQELEKRMAE VEAQKAQQLEEARLQEEEQK
  KEELARLRRELVHKANPIRKYQGLE IKSSDQPLTVPVSPKFSTRFHCTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency

Gene Information

• Gene Name: TPX2 TPX2 microtubule nucleation factor [ Homo sapiens (human) ]
• Official Symbol: TPX2
• Synonyms: DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• Gene ID: 22974
• mRNA Refseq: NM_012112.5
• Protein Refseq: NP_036244.2
• MIM: 605917
• UniProt ID: Q9ULW0