Active Recombinant HCMV (strain Merlin) gB protein, His-tagged

• Cat.No.: gB-1491H
• Product Overview: Recombinant HCMV (strain Merlin) gB protein(Ser25-Glu698), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: HCMV (strain Merlin)
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4.
• Bio-activity: Immobilized HCMV (strain Merlin) Glycoprotein B (gB), His Tag at 1 μg/mL (100 μL/well) can bind Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 with a linear range of 0.2-8 ng/mL.Immobilized Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 at 1 μg/mL (100 μL/well) can bind HCMV (strain Merlin) Glycoprotein B (gB), His Tag with a linear range of 1-39 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 78.7 kDa. The protein migrates as 105-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser25-Glu698
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.