| Description : |
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
FLAG |
| Bio-activity : |
ACE2 Human, HEK293 Recombinant binds with high affinity to the Spike S1RBD protein of the virus SARS-CoV-2.
ACE2 Human, HEK293 Recombinant was coated on an ELISA plate at 1μg/ml overnight at 4 centigrade. Recombinant SARS-CoV-2 Spike Protein S1RBD was added (starting at a concentration of 500 ng/mL with a two-fold serial dilution) during one hour at RT and the interaction was then detected using an anti-human IgG (HRP). |
| Molecular Mass : |
~110kDa (SDS-PAGE)
~240kDa (Dimer, by SEC) |
| Protein length : |
18-740 |
| Endotoxin : |
<0.01 EU/μg (LAL test). |
| Purity : |
≥ 95% |
| Applications : |
Cell culture and/or animal studies, In vitro, COVID-19 |
| Storage : |
Stable for at least 6 months after receipt when stored at -20 centigrade. After opening, prepare aliquots and store at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Storage Buffer : |
1 mg/mL after reconstitution. Lyophilized. Contains PBS. |
| Reconstitution : |
Reconstitute with 50 μL endotoxin-free water. |
| Shipping : |
Shipped on ice packs. |
