Active Recombinant Human Activin B, His-tagged

• Cat.No.: Activin B-16H
• Product Overview: recombinant Human Activin B was produced by transient expression in Nicotiana plant.

Specification

• Description: Activin B is a non-glycosylated cytokine that belongs to the TGF? superfamily. It is involved in a wide variety of biological processes including hematopoiesis, immune response, neural cell differentiation, wound repair and morphogenesis. The Activins form homodimers (e.g. AA; BB) and heterdimers (e.g. AB) and can activate the Smad pathway by signaling through heterodimeric typeI/typeII serine/threonine kinase transmembrane receptors. Activins are also important for production and regulation of several hormones such as FSH, Prolactin, ACTH and oxytocin. Activin B is synthesized by a wide spectrum of cells including bone marrow cells, skin cells, vascular smooth muscle cells and cells of the reproductive system.
• Source: Nicotiana plant
• Species: Human
• Tag: His
• Form: Lyophilized from Tris HCl at pH 7.4
• Bio-activity: Bioactivity is assayed by measuring the ability of recombinant human Activin B to inhibit proliferation of mouse plasma-cytoma cell line (MPC-11). The ED50 value for this effect is typically below 5 ng/ml.
• Molecular Mass: Single chain of 14 kDa. Recombinant human Activin B contains a protein chain of 113 amino acids and a 10 aa Histidine-based tag for a total length of 123 aa.
• Endotoxin: Endotoxin level is less than 0.05 EU/ug as measured by LAL method.
• Purity: Greater than 95% by SDS page analysis.
• Storage: The lyophilized protein, though stable at 2-8°C in the short term, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 50 μg/ml. Due to the protein nature, dimmers and multimers may be observed. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Reviews

09/05/2022

The protein product's efficacy has been validated by numerous independent research studies, solidifying its reputation as a highly effective reagent.

11/23/2021

The company values customer satisfaction and has shown a commitment to providing exceptional support throughout our interactions.

04/26/2021

The company employs rigorous quality control measures to ensure batch-to-batch consistency, guaranteeing reliable and reproducible outcomes.

07/25/2019

The protein product is produced using advanced manufacturing processes and stringent quality standards, ensuring its integrity and reliability.

07/03/2017

We have never experienced any issues with damage or contamination during shipping, thanks to the company's careful packaging and handling procedures.

Q&As

What is the significance of the upregulation of activin-B and follistatin in IPF?

The upregulation of activin-B and follistatin in IPF is a novel finding, suggesting their involvement in the disease.

What is the purpose of developing activin B-loaded hydrogels?

The purpose of developing activin B-loaded hydrogels is to achieve sustained release of activin B over an extended period.

What does Activin B induce in BMSCs?

Activin B induces stress fiber formation and migration of BMSCs.

How long does the sustained release of activin B from the hydrogels last?

The sustained release of activin B from the hydrogels lasts for over 5 weeks.

What benefits have been observed with the activin B-loaded hydrogels?

The activin B-loaded hydrogels have shown substantial cellular protection and behavioral improvement.

In what way could activin inhibition be useful in IPF?

Activin inhibition could be useful in reducing the alveolar cellular response to injury in IPF.

Does combining serum activin B concentration with free β-hCG improve the diagnostic potential for differentiating tubal ectopic pregnancy from normal pregnancy?

Yes, combining serum activin B concentration with free β-hCG improves the diagnostic potential for distinguishing tubal ectopic pregnancy from normal pregnancy.

Is activin B found naturally in the body?

Yes, activin B is produced endogenously in the body and is present in various tissues and fluids, including follicular fluid.

What is the difference in serum activin B concentration between tubal ectopic pregnancy (tEP) and normal pregnancy?

The serum activin B concentration is lower in tubal ectopic pregnancy compared to normal pregnancy.

Is serum activin B concentration reliable in distinguishing tubal ectopic pregnancy from intrauterine miscarriage (IUM)?

Serum activin B concentration cannot reliably distinguish tubal ectopic pregnancy from intrauterine miscarriage (IUM).

Can serum activin B concentration differentiate tubal ectopic pregnancy from normal pregnancy with high accuracy?

Serum activin B concentration can discriminate tubal ectopic pregnancy from normal pregnancy with moderate accuracy.

What condition or disease is being targeted with the activin B-loaded hydrogels?

The activin B-loaded hydrogels are being considered as a therapeutic strategy for Parkinson's disease.

What is activin B?

Activin B is a member of the activin family, which is a group of growth and differentiation factors involved in various biological processes.

How does the activity of purified activin B compare to other activins?

The purified activin B showed lower activity compared to other activins in various assay systems, such as follicle-stimulating hormone secretion and gonadotropin receptor expression.

Is activin inhibition effective for antifibrotic therapy in IPF?

No, it indicate that activin inhibition is not an efficient tool for antifibrotic therapy in IPF.

What are the implications of the activin B-follistatin complex in porcine follicular fluid?

In porcine follicular fluid, the activin B-follistatin complex was found to be present in equimolar amounts, suggesting a tight interaction between the two proteins.

What is the potential of the activin B-loaded hydrogels in the context of Parkinson's disease?

The findings suggest that the activin B-loaded hydrogels have the potential to be an effective therapeutic strategy for Parkinson's disease, based on the observed cellular protection and behavioral improvement.

Can activin-B and follistatin levels serve as biomarkers of IPF?

Yes, activin-B and follistatin levels may be useful as biomarkers for IPF.

What are the biological functions of activin B?

Activin B has been implicated in various biological functions, including embryonic development, cell differentiation, tissue repair, and regulation of hormone secretion.

How does activin B form a complex with follistatin?

Activin B can bind to follistatin, an activin-binding protein, to form a complex. This interaction helps regulate the activity and availability of activin B.